How can you identify bacteria carrying a plasmid that contains a fragment of the Drosophila DNA?

An ampicillin-resistant tetracycline-resistant plasmid is cleaved with EcoRI which cuts within the Show more An ampicillin-resistant tetracycline-resistant plasmid is cleaved with EcoRI which cuts within the tetracycline gene. The cut plasmid is ligated with EcoRI-digested Drosophila DNA to prepare a genomic library. This DNA is then used to transform E. coli K12. A) Under what conditions should you grow bacteria in order to select for transformed cells (those that have taken up a plasmid)? B) How can you identify bacteria carrying a plasmid that contains a fragment of the Drosophila DNA? C) How can you explain the presence of colonies that are resistant to both antibiotics? Show less

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